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Surveillance and identification of intercepted ticks carried by wool imported from Kyrgyz Republic to Xinjiang Uygur Autonomous Region, China

WANG Ke-ke, HE Yu-ping, WANG Qiang, LIN Ying-zheng, CHEN Rui-hua, WANG Yan, SHI Qian, DENG Yao-hua, XU Xin-feng, CHEN Zhi-fei, LI Shu-qing

Abstract （351）

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Objective To monitor and identify the ticks carried by wool imported from Kyrgyz Republic to China, and to prevent the transmission of harmful vectors through international trade. Methods Sixteen batches of wool imported from Kyrgyz Republic at Turgart port, Xinjiang Uygur Autonomous Region, from September 2017 to June 2018 were examined to detect ticks. The detected ticks were subjected to morphological identification. Genomic DNA was extracted from the ticks, and the mitochondrial 16S rRNA gene, 18S rRNA gene, and cytochrome C oxidase subunitⅠgene were amplified and sequenced to construct a phylogenetic tree using MEGA 7.0 software for molecular biological identification. Results Sixteen batches of wool were imported from Kyrgyz Republic, and ticks were detected in 11 batches of the imported wool. A total of 39 ticks were detected, including 1 live tick. They belonged to 5 species of 4 genera in 2 families as identified by morphological and molecular phylogenetic analysis: Ornithodoros lahorensis, Hyalomma asiaticum, Dermacentor marginatus, Haemaphysalis punctata, and H. sulcata. There were 30 ticks of H. sulcata, as the predominant species (76.9% of the total number) of intercepted ticks carried by the wool imported from Kyrgyz Republic. The live tick was a nymph of O. lahorensis. Conclusion The intercepted ticks are probably detected in wool imported from Kyrgyz Republic, which are mainly H. sulcata. The live nymph of O. lahorensis was intercepted for the first time. Therefore, the quarantine of ticks carried by wool imported from Kyrgyz Republic should be strengthened to prevent vector invasion and transmission of harmful diseases.

2020, 31 (1): 41-45. doi: 10.11853/j.issn.1003.8280.2020.01.009

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Surveillance of intercepted ticks carried by wool imported from South Africa and identification of Rhipicephalus evertsi evertsi

LI Shu-qing, WANG Qiang, LIN Ying-zheng, DENG Yao-hua, XIA Qian, CHEN Zhi-fei, WANG Yan, ZHANG Qiang, XUE Jun-xin, WANG Qiao-quan, HE Yu-ping

Abstract （294）

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Objective To quarantine and identify the ticks carried by wool imported from South Africa to China, and to prevent the transmission of harmful parasites through international trade. Methods Thirty-one batches of wool imported from South Africa at Shanghai port from January 2016 to August 2018 were examined to detect ticks. The detected ticks were subjected to morphological identification. Genomic DNA was extracted from the ticks, and the mitochondrial 16S rRNA gene and cytochrome C oxidase subunit I gene were amplified and sequenced for molecular biological identification. Results Thirty-one batches of wool were imported from South Africa at Shanghai port from January 2016 to August 2018. Ticks were observed in 8 batches of the imported wool, with a positive detection rate of 25.8%. They belonged to 8 species and 3 genera as identified by morphological and molecular biological methods. There were 15 ticks of Rhipicephalus evertsi evertsi, as the predominant species (53.6% of the total number) of intercepted ticks carried by the wool imported from South Africa; there was no distribution record of this tick species in China. Conclusion The intercepted ticks carried by wool imported from South Africa are mainly Rh. e. evertsi, which can transmit multiple animal diseases including babesiosis.

2019, 30 (4): 414-417. doi: 10.11853/j.issn.1003.8280.2019.04.013

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Identification and traceability of an intercepted tick from imported dog at Shanghai port

WANG Qiang, HE Yu-ping, WU Cheng-yun, WANG Qiao-quan, WANG Yan, LIN Ying-zheng, CHEN Zhi-fei, ZHANG Qiang, DENG Yao-hua, ZHANG Guan-nan, CAO Yu-fan, TANG Zhi-fang, LI Shu-qing

Abstract （310）

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Objective To identify the unknown species of ticks carried by the dog imported from Germany to China. Methods Use morphological identification, and extract the genomic DNA from the ticks, amplify the mitochondrial 16S rRNA sequences and sequencing. Blast in the NCBI, to identify the species of the ticks collected from Shanghai port on November 28, 2016. Results Morphological analysis revealed that the tick was in accordance with the morphological characteristics of Haemaphysalis. The sequence analysis results showed that the measured gene of this tick was 98% identical to the mitochondrial gene sequence of Ha. flava Neumann, 1897 which was widely distributed in Palaearctic and Oriental region. Conclusion This tick is Ha. flava, and infected the dog after entry.

2018, 29 (4): 340-343. doi: 10.11853/j.issn.1003.8280.2018.04.004

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Survey on Borrelia bacteria in rodents from Bayannur, Inner Mongolia Autonomous Region, China

Wulantuya, Gaowa, YIN Xu-hong, CAO Min-zhi, GUO Sheng-chun, WANG Qiang, Arionjergal, LIU Mei-qin

Abstract （271）

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Objective To examine the infection rate and genotype of Borrelia sp. among rodents, we surveyed rodent samples from Bayannur region of Inner Mongolia Autonomous Region, and to provide basic information of epidemic prevention and risk assessment for local area. Methods During 2015-2016, rodent samples were collected in Bayannur region by the trap-night method, and rodent spleen DNA samples were examined using specific PCR to detect the Borrelia sp. Results A total of 480 rodents were collected from Bayannur, and Meriones meridianus accounted for 174, and M. unguiculatus 124. The results showed that both were the predominant species in these areas. Borrelia sp. were identified from 8 (1.67%)when amplified by PCR targeting flaB gene and 16S rDNA, including 6 cases of M. unguiculatus and 2 cases of Phodopus sungorus, the positive rate were 7.14% and 4.84%, respectively. Sequence analysis of the amplification products showed identification of B. garinii and B. afzelii with 100% homology, respectively. Conclusion In this study, we confirmed the B. garinii and B. afzelii were main pathogens transmitting among rodents in Bayannur region, it is necessary to implement monitoring and prevention of Lyme diseases.

2018, 29 (3): 239-241. doi: 10.11853/j.issn.1003.8280.2018.03.005

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Experimental study on the killing effects of permethrin diehlorobenzene on fleas on spermophilus dauricus

WANG Zhi-yu, GAO Wen-lin, SHI Xian-ming, CHEN Yong-jiang, DU Guo-yi, NING Zhi-bo, WU Xiao-ming, WANG Qiang, LIU Hai-xiang

Abstract （602）

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2012, 23 (3): 272-272.

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Study on the parasitical fleas on Myospalax aspalax bodies and their epidemiological significance

WU Xiao-ming, SHI Xian-ming, NING Zhi-bo, GAO Wen-lin, WANG Qiang, WANG Wei-dong, CHEN Yong-ming, YANG Shun-lin, WANG Zhi-yu, HU Le-le

Abstract （750）

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Objective To investigate the species, composition and infection with Yersinia pestis of parasitical fleas on Myospalax aspalax bodies and their role in the epidemic of rodent plague in Hebei province, providing a scientific basis for the better control of rodent plague. Methods Parasitical fleas on the bodies of M. aspalax were collected and classified with Y. pestis isolated. Results Nine species of fleas belonging to 7 genera and 3 families were found in the present study. No Y. pestis was isolated from the 111 M. aspalax and the cultures of 715 fleas in 68 groups by microbiological tests, with the Y. pestis specific antibody negative in 88 serum samples from M. aspalax. Conclusion There are a great number of fleas on the body of M. aspalax that can be infected with Y. pestis naturally, with Amphipsylla daea being the dominant parasitical flea, which may be involved in plague epidemics.

2012, 23 (2): 154-155.

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Selective tests on Myospalax aspalax baits in laboratory

SHI Xian-ming, WANG Qiang, GAO Wen-lin, WU Xiao-ming, NING Zhi-bo, WANG Wei-dong, WANG Zhi-yu

Abstract （863）

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Objective To identify palatable baits for Myospalax aspalax for the basis of poisonous baits during the pairing season. Methods M. aspalax were fed with different baits such as carrots, potatoes, sweet potatoes, corns, peanuts and naked oats in laboratory for comparison of palatability. Results The palatability index of carrots to potatoes, sweet potatoes, corns, peanuts and naked oats were 0.65, 0.36, 0.39, 0.25 and 0.44 when feeding alone, and 0.50, 0.90, 0.01, 0.21 and 0.13 when group feeding, respectively. Carrots were the best bait, followed by potatoes and sweet potatoes, while corns, peanuts and naked oats were of poor palatability. Conclusion Carrots, potatoes and sweet potatoes are good ingredients for the preparation of poisonous baits for killing M. aspalax.

2011, 22 (6): 538-540.

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Strengthen the integrated control to plague and increase emergency control ability of Hebei province

WANG Zhi-Yu, GAO Wen-Lin, WU Xiao-Ming, NING Zhi-Bo, WANG Qiang

Abstract （1330）

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【Abstract】 Carry out the plague prevention and control measures, increase the emergency handing ability of professionals, prevent and control effectively the occurrence of plague and assure the public health security of country according to the epidemic situation of plague in Hebei province trend and its geographic position by making the plague integrated control measure, publicizing the knowledge of killing rats, the evaluation of control efficacy, professionals training and actual simulated training.

2009, 20 (2): 173-174.